h a l f b a k e r yAlas, poor spelling!
add, search, annotate, link, view, overview, recent, by name, random
news, help, about, links, report a problem
browse anonymously,
or get an account
and write.
register,
|
|
|
Which sounds better £46 or £115.83?
Now the NMRM Corp tie-in with DNA-WPR*, you need
only
take a swab, send the sample to a lab in Knoxville,
Tenn.
and have peace of mind!!!
Later edit - Ah, I forgot to include the actual point of
the idea. It's expensive to get DNA tests for ancestry
websites, so this might be a cheap way to do it.
(exchange rates are a ruff calculation)
(*DNA World Pet Registry)
Please log in.
If you're not logged in,
you can see what this page
looks like, but you will
not be able to add anything.
Destination URL.
E.g., https://www.coffee.com/
Description (displayed with the short name and URL.)
|
| |
There's an even cheaper way. Just walk into any police
station and say "I'd just like to mention that I was in no way
involved in this recent spate of murders", and they'll have you
profiled in no time. |
|
| |
Full genome? The police have enough paperwork. |
|
| |
Probably not, but then again dog testing kits only cover a
fraction of the [canine] genome. |
|
| |
Ah, I forgot to include the actual point of the idea.
It's expensive to get DNA tests for ancestry websites,
so this might be a cheap way to do it. |
|
| |
But, finding out me and Lassie have 57 points of
congruence isn't really that useful. |
|
| |
I was dreaming we can get a better price and speed by getting an uncoiled chromatid to flow, in a microfluidic channel , past a reader section, measuring the nucleotide cogs. Attaching a large molecule to one end of the chromatid and by using gravity, might 'train' it's length down a column through some sort of base discriminating sensor. Position and base relative to the chromatid would be maintained. |
|
| |
Measuring the cogs is the hard part. However, Oxford
Nanopore's execrable technology sort of does this (though not
for whole chromosomes, and with abysmally low accuracy). |
|
| |
After a cursory look, my initial feeling is the electronic state of the nanopore needs to be known more accurately to get a better read. I suggest, some molecular groups attached to the nanopore with their own reading layer to help with the a baseline. Just more data to get a better look. |
|
| |
The positional charges on the DNA atoms would be variable within a range , would they not? |
|
| |
The problem with nanopore sequencing is that the pore is
several nucleotides long. Hence, the signal at any instant is
sort of averaged over several bases. |
|
| |
What happens when I get the results and it shows I'm
prone to hard pad? |
|
| |
[not_morrison_rm] Nothing a good soak/whiskey can't fix but check your not feeling an ill temper. |
|
| |
[Max]Is several still small enough to be listed? It is interesting trying to read a changing section of a flow to try and determine subunits.
I did have another wonder, whether light or another energy form could used lightly to condition the read environment so the nucleotides have a more determining signal. |
|
| |
// several still small enough to be listed?// I think it's
something like 5 or 10. You can recover sequence data, but
the raw error rate is something horrendous like 10%, which
makes it useless for many applications. |
|
| |
//whether light or another energy form could used lightly to
condition the read environment so the nucleotides have a
more determining signal// I have no idea what that means. |
|
| |
Doesn't the signal have to range more widely and cleanly to remove raw read errors? chilling a magnet will will give a stronger field. |
|
| |
Is there a way of accentuating the charges to give a better resolve? Make the strand flow through an environmental change to which each base reacts with a stronger difference to compared to the others. Magnetism, light field, temperature. It would be good if it was like a photocopier, impart charge to strand, print it off at sensor. |
|
| |
I must say, great work having a mechanism that can capture a strand and flow it's long length through an known volumetric space. It's just a matter of time before the reader becomes accurate enough. |
|
| |
//accentuating the charges// it's not based on charge per se.
Instead, they measure the current flowing through the pore;
that current is reduced by the DNA, and the amount of
reduction depends on which bases are in the pore. |
|
| |
So its all about the base? |
|
| |
Doesn't seem like any treble at all, then. |
|
| |
There's only 10% tremble when the DNA rubs off with some of the charge. |
|
| |
I still think, because of DNA's sole purpose, a near 100% accurate read should be possible. |
|
| |
My hope is for a stack of carbon nanosheets that can have a multiple reads as the DNA spirals through even though base gap is 0.34 nm or 3.4 Å and carbon sheets range from 1 to 100nm plus insulator distance. Theoretically the gap between reader sheets should not matter. |
|
| |
Though if the animal DNA full genome sequence price drops I will use that. |
|
| |