vast numbers of blastocysts on a tape array have one of their cytes laser zapped then laser tweezers guide the contents to an area with visible gene probes to characterize the pre embryos The three meters of tissue culture tape would be spooled with wide areas permitting the passage of nutrient fluid on a freezable roll
Human Microsort (link) is around 3 to 5k per cycle yet the Non FDA version that is not directed at humans is just a few hundred dollars or less I think producing the blastocysts tape would be less than thirty times that of making thirty gene arrays thus near 3k
The Economist says that US spent near 140k per child to maturity Making Blastocyst choice a covered item approaches guaranteeing a beneficial outcome at less than 7 months of the amount of government funds per child It also prevents birth defects
The components of this are supported online
Make vast numbers of blastocysts goes with the technology of using progenitor cytes to make oocytes
large arrays of living tissue dots as well as microfluidic sortation of cytes is supported with the multiday survival of multicentimeter wide neural tissue samples as well as ordinary gamete sorting technology
the use of optical force manipulation on blastocysts is published
fluorescent gene probes are published
Now the English version Fertlity clinics recently have a procedure where they take just one cyte of a developing blastocyst then do PCR to see what genes it has People then choose which of a number of embryos to develop then implant to become babies
Our version creates then screens about a million blastocysts with microfluidics automatically to find a preferred genome
When you think about it just choosing a top blastocyst per hundred on each of three genetic characteristics would require a million blastocysts if the genes were mathematically nonlinked Using multiple passages of egg fusion to progenitor cytes to eggs you can get seven characteristics at the top blastocyte per hundred Thus if you are going with kindness genes AVPR1A as well as happiness genes 5-HTTLPR as well as any number of cleverness genes among them CHRM2 all at once as well as beauty genes like DOT1L a bunch of blastocysts are to be screened That could matter to couples as they have mutually complementary genes yet they also would like the preferred assortment of genes to make a baby
Optical tweezers use the force of light to move microscopic objects around This is published as being of use during IVF (link) This idea uses a laser to zap just one cyte of a multicyte blastocyst then move the cytoplasm plus nucleus towards a preprinted dot of optically visible dna probes plus reagent microbeads the DNA of each blastocyst is then characterized on a few dozen or hundred genes of value
The film this is based on is similar to the tissue slice of neurology Neurology researchers frequently make a millimeter thick multicentimeter wide flat neural slice that they keep alive for several days Thus rather ike a spool of tape or film a long wide likely tissue cultured tissue bed hosts the blastocysts There is material that suggests blastocysts are as good or better at surviving than many tissues notably from their ability to be frozen then thawed
a recent genome array product has about a million characterization sites on an area I perceive as less than 7 square centimeters Thus the number of blastocysts per square centimeter describes the screening volume a thousand blastocysts per square centimer on a tape 3 cm wide 300 cm long host about a million blastocysts The three meters of tissue cultured tape would be spooled with wide areas permitting the passage of nutrient fluid on a freezable roll
I don't know if having a computer controlled laser nudge a blasctocyst then blend chemicals then read a tape full of frozen blastocysts is as effective as a growth temperature tissue tape
When the computer controlled mechanism reads the tape it makes a gap at the membrane of one of the blastocysts component cytes This is currently a normal procedure
The optical force effect is used to move the cytoplasm with nucleus to the gene probe area which has hundreds of possible gene matches avialable As necessary the laser also pops microbeads of DNA unspooling reagents Once the optical probe has reacted the laser then pops a microbead of EDTA or laser forms a separation channel between the living blastocyst plus the chemistry region to tidily maintain the blastocysts growth environment
Microfluidic sortation of gametes according to sex produces large numbers of gametes that can be used vaginally to the uterus with IUI That is near several million gametes per milliliter of sorted material Each neural tissue slice preparation maintains billions of neurons at the surface of the culture medium Print patterning blastocysts onto a living tissue tape with a number of microbeads of reagents is less dense than current printed gene arrays-- beanangel, Oct 23 2009 laser optical tweezers used with gametes http://humrep.oxfor...ent/short/10/7/1761 [beanangel, Oct 23 2009] Gene microarrays at wikipedia http://en.wikipedia.org/wiki/Gene_array [beanangel, Oct 23 2009] (?) microsort http://www.microsort.net/fees.php [beanangel, Oct 23 2009] a million or 1.8 million genetic variants detected with a current genetic microarray http://www.affymetr...ome_wide_snp_6.affx [beanangel, Oct 23 2009] 'angel messes with 'nads once again.-- neelandan, Oct 24 2009 So very much more no than the peeing-in-a-shoe idea.-- MaxwellBuchanan, Oct 24 2009 We have been doing this for Milennia.-- 8th of 7, Oct 24 2009 Where do these vast numbers of genetically different blastocysts come from?-- wjt, Oct 24 2009 //vast numbers of blastocysts on a tape array have one of their cytes laser zapped then laser tweezers guide the contents to an area with visible gene probes to characterize the pre embryos// <George, 'Thick as a Whale Omelette', Prince of Wales> "Nope. Didn't get any of that." </GTAAWOPOW>-- DrBob, Oct 26 2009 random, halfbakery